當前位置:  首頁 >> 最新重要論文

最新重要論文

Structural insights into assembly, operation and inhibition of a type I restriction–modification system, Nat Microbiol, 1 Jun 2020

發布時間:2020年06月01日

Nature Microbiology, 1 June, 2020, DOI:https://doi.org/10.1038/s41564-020-0731-z

Structural insights into assembly, operation and inhibition of a type I restriction–modification system

Yina Gao, Duanfang Cao, Jingpeng Zhu, Han Feng, Xiu Luo, Songqing Liu, Xiao-Xue Yan, Xinzheng Zhang & Pu Gao

Abstract

Type I restriction–modification (R–M) systems are widespread in prokaryotic genomes and provide robust protection against foreign DNA. They are multisubunit enzymes with methyltransferase, endonuclease and translocase activities. Despite extensive studies over the past five decades, little is known about the molecular mechanisms of these sophisticated machines. Here, we report the cryo-electron microscopy structures of the representative EcoR124I R–M system in different assemblies (R2M2S1, R1M2S1 and M2S1) bound to target DNA and the phage and mobile genetic element-encoded anti-restriction proteins Ocr and ArdA. EcoR124I can precisely regulate different enzymatic activities by adopting distinct conformations. The marked conformational transitions of EcoR124I are dependent on the intrinsic flexibility at both the individual-subunit and assembled-complex levels. Moreover, Ocr and ArdA use a DNA-mimicry strategy to inhibit multiple activities, but do not block the conformational transitions of the complexes. These structural findings, complemented by mutational studies of key intermolecular contacts, provide insights into assembly, operation and inhibition mechanisms of type I R–M systems.

文章鏈接:https://www.nature.com/articles/s41564-020-0731-z

相關報道:http://www.8391397.live/kyjz/zxdt/202006/t20200603_5601872.html

 

 

    附件下載:
赚钱的作弊码 大圣配资靠谱吗 河南泳坛夺金20分钟 解排三太湖字谜 幸运28预测上pc28点gd 上海快三每天开奖时间 上海快3走势图200分布图 福建快3下载安装 expma超短线选股战法 上海天天彩选4基本走势图 安徽省快3开奖走势图